Pdcd1 Knockout cell line (MC38)

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Pdcd1 Knockout cell line (MC38) Order

Pdcd1 Knockout cell line (MC38)
Catalog#
YKO-M241
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Pdcd1 Knockout cell line (MC38)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Pdcd1 Knockout cell line (MC38)
Cell Morphology
Epithelial-like, Mixed: adherent and suspensio
Passage ratio
1：2-1：4
Species
Mouse
Gene Name
Pdcd1
Gene ID
18566
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript Pdcd1-201)
Validation: PCR+Sanger Sequencing
Pdcd1 Knockout cell line (MC38) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:MC38		 STR Info (Cell bank)
Cell Line:MC-38[EXPASY kerafast]
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 15.016.015.016.0
4-2 20.320.3
6-7 15.016.015.016.0
19-2 13.013.0
1-2 19.018.019.020.0
7-1 26.226.2
8-1 16.016.0
1-1 16.017.015.016.017.0
3-2 13.014.015.014.015.0
2-1 16.016.0
15-3 22.323.322.323.3
6-4 18.018.0
13-1 17.017.0
11-2 16.016.0
17-2 15.016.015.016.0
12-1 17.017.0
5-5 16.017.016.017.0
X-1 27.027.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Pdcd1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MC38 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Pdcd1 gene knockout Pdcd1 Knockout cell line (MC38) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Pdcd1 Knockout cell line (MC38)
Pdcd1 Knockout cell line (MC38)
Catalog#
YKO-M241
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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